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adherent tumor cells  (Thermo Fisher)


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    Thermo Fisher adherent tumor cells
    Adherent Tumor Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/adherent+tumor+cells/Trypsin/pm41168822-164-4-11
    Average 99 stars, based on 1 article reviews
    adherent tumor cells - by Bioz Stars, 2026-09
    99/100 stars

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    Article Title: Genome-wide association, single-cell, and spatial transcriptomics analyses reveal the role of the STK24-expressing positive cells in LUAD progression and the tumor microenvironment, identifying STK24 as a potential therapeutic target.
    Article Snippet: We performed digestion of adherent tumor cells (with 0.25% trypsin (Trypsin, Gibco) for 1 min, terminated by adding complete RPMI 1640 medium (supplemented with 10% fetal bovine serum)), collected the cells into a centrifuge tube, centrifuged, and counted the cells.

    Article Title: Co-Stimulatory Bispecific Antibodies Induce Enhanced T Cell Activation and Tumor Cell Killing in Breast Cancer Models
    Article Snippet: Adherent tumor cells (MCF-7, HT-1080/FAP) were trypsinized (0.05% trypsin/EDTA; Gibco) and collected.

    Article Title: CD137 Stimulation Enhances Cetuximab-Induced Natural Killer: Dendritic Cell Priming of Antitumor T-Cell Immunity in Patients with Head and Neck Cancer
    Article Snippet: Adherent tumor cells were detached by warm Trypsin-EDTA (0.25%) solution (Invitrogen, Carlsbad, CA).

    Article Title: Genome-wide association, single-cell, and spatial transcriptomics analyses reveal the role of the STK24-expressing positive cells in LUAD progression and the tumor microenvironment, identifying STK24 as a potential therapeutic target
    Article Snippet: We performed digestion of adherent tumor cells (with 0.25% trypsin (Trypsin, Gibco) for 1 min, terminated by adding complete RPMI 1640 medium (supplemented with 10% fetal bovine serum)), collected the cells into a centrifuge tube, centrifuged, and counted the cells.

    Article Title: CD137 stimulation enhances cetuximab induced natural killer (NK): dendritic cell (DC) priming of anti-tumor T cell immunity in head and neck cancer patients
    Article Snippet: Adherent tumor cells were detached by warm Trypsin-EDTA (0.25%) solution (Invitrogen, Carlsbad, CA).

    Article Title: Cetuximab-activated natural killer (NK) and dendritic cells (DC) collaborate to trigger tumor antigen-specific T cell immunity in head and neck cancer patients
    Article Snippet: Adherent tumor cells were detached by warm Trypsin-EDTA (0.25%) solution (Invitrogen, Carlsbad, CA).

    Article Title: Nimotuzumab Induces NK Cell Activation, Cytotoxicity, Dendritic Cell Maturation and Expansion of EGFR-Specific T Cells in Head and Neck Cancer Patients
    Article Snippet: Adherent tumor cells were detached by warm Trypsin–EDTA (0.25%) solution (Invitrogen).



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    Preclinical efficacy and antitumor activity of CTX130. A, Proliferation of CTX130 versus CD70 + CAR T cells. B, CTX130 and CD70 + anti-CD70 CAR T-cell cytotoxicity as a percentage of cell lysis after repeated challenges with CD70 + <t>A498</t> cells. Data points represent a single measurement. C, CTX130 cytotoxicity toward CD70 high (A498), CD70 low (ACHN), and CD70 − (MCF7) cell lines. Results from cells treated with CTX130 are shown with solid circles, and results from cells treated with unedited T cells are shown with open squares. The graph shows the mean ± SD from 3 technical replicates with increasing ratios of CTX130 or unedited T cells to tumor cells (0.125:1 to 4:1). D, Antitumor activity of CAR T cells in an RCC xenograft model. Mice were either left untreated ( n = 5) or injected with CTX130 ( n = 5) or CD70 + anti-CD70 CAR T cells ( n = 4). Each point represents the mean tumor volume ± SEM. E, Disruption of the PD-1 checkpoint gene is detrimental to CTX130 CAR T-cell function in a xenograft rechallenge model. Following the first injection of tumor cells, mice ( n = 5 per group) were left untreated (open circles) or treated with CTX130 (open squares) or PD1 − CTX130 CAR T cells (open triangles). At the day 25 rechallenge, fresh tumor cells were injected into the left flank of treated mice (CTX130-treated and PD1 − CTX130-treated animals represented by solid circles and solid triangles, respectively) and a new control group (open lozenges). Each point represents the mean tumor volume ± SEM. F, Mortality due to GvHD in mice ( n = 30 per group) treated with unedited T cells or low (20 million cells/mouse) or high (40 million cells/mouse) doses of CTX130. CAR, chimeric antigen receptor; CD, cluster of differentiation; EP, electroporation; GvHD, graft versus host disease; KO, knockout; PD-1, programmed cell death 1; RCC, renal cell carcinoma.
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    Preclinical efficacy and antitumor activity of CTX130. A, Proliferation of CTX130 versus CD70 + CAR T cells. B, CTX130 and CD70 + anti-CD70 CAR T-cell cytotoxicity as a percentage of cell lysis after repeated challenges with CD70 + A498 cells. Data points represent a single measurement. C, CTX130 cytotoxicity toward CD70 high (A498), CD70 low (ACHN), and CD70 − (MCF7) cell lines. Results from cells treated with CTX130 are shown with solid circles, and results from cells treated with unedited T cells are shown with open squares. The graph shows the mean ± SD from 3 technical replicates with increasing ratios of CTX130 or unedited T cells to tumor cells (0.125:1 to 4:1). D, Antitumor activity of CAR T cells in an RCC xenograft model. Mice were either left untreated ( n = 5) or injected with CTX130 ( n = 5) or CD70 + anti-CD70 CAR T cells ( n = 4). Each point represents the mean tumor volume ± SEM. E, Disruption of the PD-1 checkpoint gene is detrimental to CTX130 CAR T-cell function in a xenograft rechallenge model. Following the first injection of tumor cells, mice ( n = 5 per group) were left untreated (open circles) or treated with CTX130 (open squares) or PD1 − CTX130 CAR T cells (open triangles). At the day 25 rechallenge, fresh tumor cells were injected into the left flank of treated mice (CTX130-treated and PD1 − CTX130-treated animals represented by solid circles and solid triangles, respectively) and a new control group (open lozenges). Each point represents the mean tumor volume ± SEM. F, Mortality due to GvHD in mice ( n = 30 per group) treated with unedited T cells or low (20 million cells/mouse) or high (40 million cells/mouse) doses of CTX130. CAR, chimeric antigen receptor; CD, cluster of differentiation; EP, electroporation; GvHD, graft versus host disease; KO, knockout; PD-1, programmed cell death 1; RCC, renal cell carcinoma.

    Journal: Cancer Discovery

    Article Title: CD70-Targeted Allogeneic CAR T-Cell Therapy for Advanced Clear Cell Renal Cell Carcinoma

    doi: 10.1158/2159-8290.CD-24-0102

    Figure Lengend Snippet: Preclinical efficacy and antitumor activity of CTX130. A, Proliferation of CTX130 versus CD70 + CAR T cells. B, CTX130 and CD70 + anti-CD70 CAR T-cell cytotoxicity as a percentage of cell lysis after repeated challenges with CD70 + A498 cells. Data points represent a single measurement. C, CTX130 cytotoxicity toward CD70 high (A498), CD70 low (ACHN), and CD70 − (MCF7) cell lines. Results from cells treated with CTX130 are shown with solid circles, and results from cells treated with unedited T cells are shown with open squares. The graph shows the mean ± SD from 3 technical replicates with increasing ratios of CTX130 or unedited T cells to tumor cells (0.125:1 to 4:1). D, Antitumor activity of CAR T cells in an RCC xenograft model. Mice were either left untreated ( n = 5) or injected with CTX130 ( n = 5) or CD70 + anti-CD70 CAR T cells ( n = 4). Each point represents the mean tumor volume ± SEM. E, Disruption of the PD-1 checkpoint gene is detrimental to CTX130 CAR T-cell function in a xenograft rechallenge model. Following the first injection of tumor cells, mice ( n = 5 per group) were left untreated (open circles) or treated with CTX130 (open squares) or PD1 − CTX130 CAR T cells (open triangles). At the day 25 rechallenge, fresh tumor cells were injected into the left flank of treated mice (CTX130-treated and PD1 − CTX130-treated animals represented by solid circles and solid triangles, respectively) and a new control group (open lozenges). Each point represents the mean tumor volume ± SEM. F, Mortality due to GvHD in mice ( n = 30 per group) treated with unedited T cells or low (20 million cells/mouse) or high (40 million cells/mouse) doses of CTX130. CAR, chimeric antigen receptor; CD, cluster of differentiation; EP, electroporation; GvHD, graft versus host disease; KO, knockout; PD-1, programmed cell death 1; RCC, renal cell carcinoma.

    Article Snippet: The A498 adherent cancer cell line was trypsinized and seeded in ATCC-recommended media (EMEM + 10% FBS) in a 6-well plate (Corning Inc.; catalog no. 3506).

    Techniques: Activity Assay, Lysis, Injection, Disruption, Cell Function Assay, Control, Electroporation, Knock-Out